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Lycopene-ε-cyclase pre-mRNA is alternatively spliced in Cara Cara navel orange (Citrus sinensis Osbeck)

Identifieur interne : 002831 ( Main/Exploration ); précédent : 002830; suivant : 002832

Lycopene-ε-cyclase pre-mRNA is alternatively spliced in Cara Cara navel orange (Citrus sinensis Osbeck)

Auteurs : Neng-Guo Tao [République populaire de Chine] ; JUAN XU [République populaire de Chine] ; Yun-Jiang Cheng [République populaire de Chine] ; Xiu-Xin Deng [République populaire de Chine]

Source :

RBID : Pascal:05-0392162

Descripteurs français

English descriptors

Abstract

Lycopene-ε-cyclase is one of the key enzymes related to α-carotene metabolism in plants. A full-length cDNA of 1300 bp encoding lycopene-ε-cyclase (Lyce) was generated from Cara Cara navel orange, a unique navel orange containing both lycopene and β-carotene in its pulp, with little or no α-carotene. The gene had a 14 bp nucleotides deletion and caused a terminal mutation. DNA sequence corresponding to the deletion region revealed that two repeats of 6 bp (AGGTGT) were flanking the region in both Cara Cara and its original variety, Washington navel oranges, but a 2 bp (AT) insertion was only found in Cara Cara which explain the alternative splicing character of the gene.


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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<div type="abstract" xml:lang="en">Lycopene-ε-cyclase is one of the key enzymes related to α-carotene metabolism in plants. A full-length cDNA of 1300 bp encoding lycopene-ε-cyclase (Lyce) was generated from Cara Cara navel orange, a unique navel orange containing both lycopene and β-carotene in its pulp, with little or no α-carotene. The gene had a 14 bp nucleotides deletion and caused a terminal mutation. DNA sequence corresponding to the deletion region revealed that two repeats of 6 bp (AGGTGT) were flanking the region in both Cara Cara and its original variety, Washington navel oranges, but a 2 bp (AT) insertion was only found in Cara Cara which explain the alternative splicing character of the gene.</div>
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